Vitex trifolia is found in tropical and subtropical regions, although vitex species can be found in temperate zones. Vitex trifolia belongs to the vitex genus and the Verbenaceae family; there are approximately 270 known species of shrubs and trees[17]. Vitex trifolia has larvicidal, healing, anti-HIV, trypanocidal and anti-tumor activity[10]. It is also used as an antibacterial, anti-inflammatory and antipyretic agent. In some countries they are also used as sedatives for rheumatism, headaches and the common cold. Vitex species used in traditional medicine to treat ailments such as wounds, allergies, asthma and body pain[15]. Collection of plant materials Fresh leaves of vitex trifolia were collected from well grown trees in Tamilnadu Agricultural University, Katuthotam, Thanjavur district, Tamilnadu. The fresh leaves were washed twice in plain water and in distilled water. The washed leaves were dried in the shade at room temperature for 15–20 days. Preparation of extracts from vitex trifolia leaves Selected organic solvents were used for the extraction of phytochemical compounds from vitex trifolia leaf materials. The shade-dried leaves are finely powdered using a blender[12]. 15 g of the powdered leaf was first extracted with petroleum ether solvent to degrease the extract using Soxhlet apparatus. The defatted powder was dried and extractions were successfully carried out with the solvents petroleum ether, benzene, acetone, ethanol and distilled water using the Soxhlet apparatus[2]. A rotary vacuum evaporator was used to evaporate the solvents and the concentrated extracts were obtained. The concentrated extracts were subjected to qualitative and quantitative tests for the identification of phytochemicals present in the leaf... in the paper medium... roids, tannins (0.521 mg) and terpinoids (0.321 mg). The ethanol extract showed the presence of alkaloids, flavonoids (0.232 mg), phenols (0.163 mg), saponins (0.123 mg), tannins (0.461 mg) and terpenoids (0.361 mg). Finally, the aqueous extract showed the presence of flavonoids (0.423 mg) and tannins (0.62 mg). The antibacterial activity of the different extracts was carried out using the disk diffusion method. The extracts showed different degrees of antibacterial activity. In Bacillus subtilis the acetonic extract of V.trifolia showed the zone of inhibition at 0.5 cm, the ethanolic extract of V.trifolia showed the zone of inhibition at 0.75 cm. In Escherichia coli the acetone extract of V.trifolia showed the zone of inhibition at 0.15 cm, the ethanolic extract of V.trifolia showed the zone of inhibition at 0.25 cm, the Dis.H2O extract of V.trifolia showed the zone of inhibition at 0.35 cm.